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. 2022 Mar 1;15:843754. doi: 10.3389/fnmol.2022.843754

FIGURE 1.

FIGURE 1

Acute itch stimuli and Chronic itch stimuli increase FOS expression in the ZIv. (A) Representative images of FOS staining in the ZI after saline (the upper panel), histamine (the middle panel), and chloroquine (the lower panel) injection. Scale bar = 200 μm. (B) Quantification of FOS-expressing neurons in response to saline, histamine, and chloroquine injection showed both histamine and chloroquine stimuli increased FOS expression in the ZIv instead of other ZI subregions. n = 3 mice per group, three sections per mouse. One-way ANOVA with post hoc LSD test for multiple comparisons. For the analysis in ZIv, F(2,6) = 17.378, P = 0.003. (C) Representative images of FOS staining in the ZI in control (the upper panel) and AD (the lower panel) mice. Scale bar = 200 μm. (D) Quantification of FOS-expressing neurons in control and AD mice showed AD increased FOS expression in the ZIv instead of other ZI subregions. n = 3 mice per group, three sections per mouse. Unpaired T-test. For the analysis in ZIv, t = –4.759, P = 0.009. ZIr, the rostral ZI; ZIv, the ventral ZI; ZId, the dorsal ZI; ZIc, caudal ZI; AD, atopic dermatitis. ∗P < 0.05, ∗∗P < 0.01.