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. 2022 Mar 1;15:843754. doi: 10.3389/fnmol.2022.843754

FIGURE 2.

FIGURE 2

Acute and chronic itch stimuli increase FOS expression in ZI PV neurons. (A–C) Representative images of FOS and PV staining in ZI after saline (the upper panel), histamine (the middle panel), and chloroquine (the lower panel) injection. (B) Representative images of FOS and PV staining in ZI in control (the upper panel) and AD (the lower panel) mice. Scale bar = 100 μm. (C) Quantification of FOS-expressing neurons in response to saline, histamine, and chloroquine injections showed both histamine and chloroquine stimuli increased FOS expression in ZI PV neurons. n = 3 mice per group, three sections per mouse. One-way ANOVA with post hoc LSD test for multiple comparisons. F(2,6) = 73.642, P < 0.001. (D) Quantification of FOS-expressing neurons in control and AD mice showed chronic itch stimuli increased FOS expression in ZI PV neurons. n = 3 mice per group, three sections per mouse. Unpaired T-test. t = –7.03, P = 0.002. ∗∗∗∗P < 0.0001, ∗∗P < 0.01.