TABLE 4.

Anti-cholera toxin B subunit antibody responses in infant rhesus macaques following inoculation with SIV_mac055 or SIV_mac385

Animal group	Animal no.	SIVa	PMPAb	Anti-cholera toxin B subunit IgG titer at the following times (wk) after virus inoculationc:
				0	2	3	4	6	8	10	12	14–16
A	29668	055	−	<100	<100	100	100	100	100	100	<100	<100d
	29669	055	−	<100	<100	<100	<100	<100	<100d
	29670	055	−	<100	<100	<100	<100	<100	<100d
B	29674	385	−	<100	400	1,600	6,400	1,600	6,400	6,400	1,600d
	29675	385	−	<100	<100	<100	<100	<100	<100	<100d
	29729	385	−	<100	<100	<100	100	<100d
C	29283	055	+	<100	<100	100	400	400	1,600	102,400	25,600	25,600
	29360	055	+	<100	<100	<100	<100	<100	<100	100	100	<100
	29419	055	+	<100	100	100	400	100	100	<100	<100	<100
D	29276	385	+	<100	100	100	100	400	400	25,600	25,600	6,400
	29278	385	+	<100	<100	400	6,400	25,600	6,400	102,400	102,400	25,600
	29279	385	+	<100	<100	<100	<100	100	100	6,400	6,400	6,400

^aAnimals were inoculated at birth with SIV_mac055 (055) or SIV_mac385 (385). 

^bGroups C and D were started on PMPA treatment 3 weeks after virus inoculation. 

^cAnimals were immunized with cholera toxin B subunit at birth (just prior to the virus inoculations), and a booster immunization was given 8 weeks later. IgG titers at the indicated times after virus inoculation were determined by anti-cholera toxin B subunit IgG-specific ELISA and are expressed as the reciprocal of the highest of fourfold dilutions (starting from a 1/100 dilution with two replicates per dilution) which gave a positive optical density above the cutoff value. Positive antibody responses (titer, ≥400) are indicated in boldface. Four uninfected control newborn macaques from a different study (27) which received the same immunizations had IgG titers of ≥6,400 at 4 weeks of age and titers of ≥102,400 at 12 weeks of age (data not shown). Blank cells indicate that samples were not available (due to animal death prior to the time point). 

^dTime point near the time of euthanasia.