Fig. 1.

Experimental setup of the isolated perfused ventilated murine lung (IPVML) model (A). A freshly isolated lung heart preparation was perfused through the pulmonary artery and ventilated through the trachea. Twenty ml of solutions with Englerin A (EA, 50, 100 nM) or equilibrated to pH 6.0, pH 5.0 and pH 4.0 were relased from a reservoir in the perfusion solution. Lung weight and tidal volumes were continously quantified by in built weighing scales or the software, respectively. Time line of the experiment (B). After 15 min perfusion EA in solution or solutions of decreased pH were released from the reservoir. The pH was continously measured in the reservoir and adjusted for 10 min, if necessary. The outlet was closed after 4 min and the perfusion solutions circulated for another 50 min in a closed circuit