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. 2022 Feb 14;96(4):1055–1063. doi: 10.1007/s00204-022-03235-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Structural formula of (−)-englerin A (EA, provided by the manufacturer, see www.carlroth.com, A) and Ca²⁺ imaging experiments of HEK 293T cells heterologously expressing TRPC4 channels (TRPC4 transfected) or mock-transfected controls after application of EA (50 nM, 100 nM) before and after 1 h perfusion in the IPVLM model. One representative experiment (n > 3 cells) out of three is shown (B–E)