PCR amplicons obtained with Rickettsia genus primers from EDTA-blood samples from two dogs in each study group on PIDs 0, 5, and 21. Dogs 182 and 193, group I (untreated infection controls), dogs 188 and 189, group II (doxycycline treated); dogs 192 and 195, group III (azithromycin treated); dogs 183 and 187, group IV (trovafloxacin treated); +, positive control (tissue culture-grown R. rickettsii); −, negative control (water).