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. 2022 Mar 14;8:114. doi: 10.1038/s41420-022-00915-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Western blot was used to verify the successful construction of GSDMD-overexpressing cell line. B Gene ontology (GO) analysis of GSDMD-interacting proteins identified by co-IP followed by mass spectrometry. C Network analysis of GSDMD interacting proteins that involved in ER-stress response. D Western blot detecting eIF2α and GSDMD following immunoprecipitation of flag-GSDMD. E Western blot detecting eIF2α and GSDMD following immunoprecipitation of eIF2α. F Western blot showing the level of phosphorylated eIF2α in the control and GSDMD-overexpression cells. G Western blot showing the level of phosphorylated eIF2α when knocking down GSDMD by siRNA.