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. 2022 Mar 1;10:862524. doi: 10.3389/fcell.2022.862524

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Copyright © 2022 Gu, Wang, Wang, Li, Liu, Lin, Yang, Zhang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exosome characterization and function. (A) Exosome-related positive-markers like CD9, CD63, HSP70, TSG101 and negative-markers like Calnexin were detected by Western blot (B) The particle size was determined by Nanosight particle analysis. (C) TEM analysis showed the cup-shaped morphology of exosomes. (D, E) Both MM-MSC conditioned medium (D) and exosomes (E) showed significant pro-proliferative roles in MM cells compared with BD-MSCs. (F) miR-483-5p expression after treatment with conditioned medium or exosomes was tested by qRT-PCR.