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. 2022 Mar 14;13:1314. doi: 10.1038/s41467-022-28934-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Scatter plots of B_net vs.: a resolution; b R_work; c R_free; d temperature; e molecular mass; f the number of aspartate/glutamate side-chain oxygen atoms; g the percentage of aspartate/glutamate residues; and h the strength of B-factor restraints applied in the final round of refinement. The Spearman’s rank correlation coefficient (ρ_S) between the two variables is reported for each plot. Source data are provided at 10.5281/zenodo.5566558.