Fig. 6. Observation of typical radiation damage features in structures with high B_net values.

wwPDB validation statistics, models and density maps for the ten structures with the highest B_net values in the PDB-REDO databank. For the eight structures with visible radiation damage artefacts in their associated electron density difference maps, a representative example is shown: for the two structures without such artefacts, the alternative causes of their high B_net and B_net-percentile values are shown instead. 2mF_obs – DF_calc maps (blue) are contoured at 1.5 rmsd; F_obs – F_calc difference density maps are contoured at ± 3.0 rmsd (green/red).