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. 2022 Mar 14;13:1310. doi: 10.1038/s41467-022-28833-4

Fig. 4. Global transcriptomic changes induced by low temperature and misregulated in the prx62-1 prx69-1 mutant are associated with PRXs and cell associated-EXTs.

Fig. 4

a Heat-map showing the hierarchical gene clustering for 1551 A. thaliana genes differentially expressed (DE) between room temperature growth (22 °C) and low temperature (10 °C) growth in wild type Col-0 and in double mutant prx62-1 prx69-1 roots. Gene Ontology analysis results depicting the top 7 most significantly enriched GO terms are shown as bubble plots on the right for the clusters of interest. DE genes in clusters 1, 2 and 4 were contrasted against all the expressed genes for GO analysis. The size of the points reflects the amount of gene numbers enriched in the GO term. The color of the points means the p-value. Relevant gene examples of specific clusters (1, 2, and 4) are listed on the left. b Expression of EXT6 and PRP1 is gradually upregulated at low temperature (10 °C) in 4 genotypes from very short RHs (Bu-2) to very long RHs (Wc-1) (RNA-seq data). TPM = Transcripts Per Kilobase Million.