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. 2022 Mar 14;13:1327. doi: 10.1038/s41467-022-28775-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Representative overview images showing c-Kit⁺ (green) cells in femoral BM at E16.5 (a), E17.5 (b) and E18.5 (c). CD31(Red); Nuclei, DAPI (blue). d, e UMAP plots showing unsupervised clustering of BM cells from E18.5 and adult femur after depletion of Lin⁺ cells (d) and the differences between the sample groups (e). f UMAP plot showing unsupervised subclustering of E18.5 and adult haematopoietic stem and progenitor cells (HSPCs). g Bar chart indicating percentage of cells in each subcluster. Cell numbers are shown on the right. h Differentially expressed genes (DEGs) comparing each subcluster of HSPC between E18.5 and adult. Blue dots, p-adjusted value < 0.001; Red dots, p-adjusted value < 0.001 and Log₂ fold change >1. Two-tailed Student’s t test. i Violin plots showing the expression of selected genes with log normalized values (y axis) for critical transcription factors and receptors in each subcluster of E18.5 and adult HSPCs.