. 2022 Mar 1;13:852733. doi: 10.3389/fphys.2022.852733

Table 2.

Number of incubated eggs (Ni), semi-transparent eggs eliminated at ED18, eggs kept in the incubator after the candling process (Ns), unhatched products—detailed as unhatched dead in shell embryos, hatched dead chicks and unhatched live in shell chicks; number of hatched chicks (Nh)—detailed as first and second grade chicks including wet chicks.

		B batch				E batch
		C	W	Ct	p	C	W	Ct	p
Incubated eggs (Ni)		3200	3200	3200	–	3200	3200	3200	–
Semi-transparent eggs		297^b	285^b	788^a	<0.001	1024^b	1036^b	1384^a	<0.001
Viable eggs (Ns)		2903^b	2915^b	2412^a	<0.001	2176^b	2164^b	1816^a	<0.001
Unhatched products	Unhatched dead	104^b	74^c	175^a	<0.001	158^b	173^b	229^a	<0.001
	Hatched dead	36	41	44	0.663	38	52	37	0.186
	Unhatched live	74^b	67^b	196^a	<0.001	39^b	41^b	96^a	<0.001
Hatchlings (Nh)		2689^b	2733^b	1997^a	<0.001	1941^b	1898^b	1454^a	<0.001
1st grade chicks	1st grade chicks	2649^b	2666^b	1919^a	<0.001	1889^b	1830^b	1358^a	<0.001
	males	1350	1320	1086	–	929	932	677	–
	females	1299	1346	833	–	960	898	681	–
2nd grade chicks	2nd grade chicks	40^b	67^a	78^a	0.002	52^b	68^b	96^a	<0.001
2nd grade chicks	wet chicks	15^b	21^b	60^a	<0.001	21^b	18^b	36^a	0.024

Ct, control group; C, cool storage; and W, warm storage. B: eggs from breeder flocks at the beginning of the laying period; E: end of the laying period. p indicates the statistical difference between groups within batches, comparing numbers relative to Ni. a, b: values not associated with common letters within batches are statistically different (p < 0.05).