Table 1. Charité Berlin E assay reagents.

Component	Volume (μl)
	Neat *	0.75	0.5
Nuclease-free water	4.5	4.75	5
4x TaqMan ® Fast Virus 1-Step Master Mix	2.5 *	2.5 *	2.5 *
E Sarbeco forward primer	0.4	0.3	0.2
E Sarbeco reverse primer	0.4	0.3	0.2
E Sarbeco probe	0.2	0.15	0.1
RNA template	2	2	2
Total reaction volume	10	10	10

^*Half of recommended assay volumes