Table 4. European Virus Archive – GLOBAL (EVAg) E gene (primers-probe set) assay reagents.

Component	Volume (µl)
	Neat
Nuclease-free water	4.3
4x TaqMan ® Fast Virus 1-Step Master Mix	2.5 *
EVA-g E Forward primer	0.5
EVA-g E Reverse primer	0.5
EVA-g E probe	0.2
RNA template	2
Total reaction volume	10

^*Half of recommended assay volume where 4X TaqMan ^® Fast Virus 1-Step Master Mix (recommended 5 µl)