Table 5. European Virus Archive – GLOBAL (EVAg) E and RdRP gene assay reagents with readily provided mix of forward and reverse primers and probes.

Component	Volume (µl)
	0.75	0.5
Nuclease-free water	2.9	3.75
4x TaqMan ® Fast Virus 1-Step Master Mix	2.5 *	2.5 *
Primer-probe mix	2.6	1.75
RNA template	2	2
Total reaction volume	10	10

^*Half of recommended assay volume where 4X TaqMan ^® Fast Virus 1-Step Master Mix (recommended 5 μl)