Table 9. Quantitative reverse transcription-PCR (qRT-PCR) cycling conditions for detection of SARS-CoV-2 RNA using four assays.

Step	Berlin Charité (E, N and RdRP)		EVAg (E and RdRp)		BGI (ORF1ab)	DAAN (ORF1ab and N)
	QuantiFast RT-PCR +R	TaqMan ® Fast Virus	QuantiFast RT-PCR +R	TaqMan ® Fast Virus	Kit component	Kit component
Reverse transcription	55°C	50°C	55°C	50°C	50°C	50°C
	20 min	5 min	20 min	5 min	20 mins	15 mins
Activation	95°C	95°C	95°C	95°C	95°C	95°C
	5 min	20 sec	5 min	20 sec	10 mins	15 mins
Denaturation	95°C	95°C	95°C 15 sec 45 cycles	95°C	95°C	94°C
	15 sec	3 sec		3 sec	15 sec	15 sec
	45 cycles	40 cycles		40 cycles	40 cycles	45 cycles
Annealing and extension	58°C	60°C	58°C	58°C	60°C	55°C
	30 sec	30 sec	45 sec	45 sec	30 sec	45 sec
	45 cycles	40 cycles	40 cycles	40 cycles	40 cycles	45 cycles