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| Component | Volume (μl) | ||
|---|---|---|---|
| 0.5X | 0.375X | 0.25X | |
| Reaction mix (Liquid A) | 8.5 | 6.4 | 4.3 |
| Enzyme mix (Liquid B) | 0.5 | 0.5 | 0.5 |
| Nuclease free water | 0 | 1.1 | 3.2 |
| RNA template | 2 | 2 | 2 |
| Total reaction volume | 10 | 10 | 10 |
