Table 4. Titration volumes of PCR reaction mix and enzyme mix from the BGI kit.

Component	Volume (μl)			Altered enzyme mix volume (μl) at 0.375X of the reaction mix
	0.5X	0.375X	0.25X	Mix 1 (0.33X)	Mix 2 (0.16X)
Reaction mix	9.3	7.0	4.7	7.0	7.0
Enzyme mix	0.8	0.8	0.8	0.5	0.25
Nuclease free water	0	0	0	0.5	0.75
RNA template	2	2	2	2	2
Total reaction volume	10	10	10	10	10