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| Component | Volume (μl) | Altered enzyme mix volume (μl)
at 0.375X of the reaction mix |
||||
|---|---|---|---|---|---|---|
| 0.5X | 0.375X | 0.25X | Mix 1 (0.33X) | Mix 2 (0.16X) | ||
| Reaction mix | 9.3 | 7.0 | 4.7 | 7.0 | 7.0 | |
| Enzyme mix | 0.8 | 0.8 | 0.8 | 0.5 | 0.25 | |
| Nuclease free water | 0 | 0 | 0 | 0.5 | 0.75 | |
| RNA template | 2 | 2 | 2 | 2 | 2 | |
| Total reaction volume | 10 | 10 | 10 | 10 | 10 | |
