TABLE 7.
Deduced amino acid sequences of PBP2x in 300 S. pneumoniae isolates.
| PBP2x group | N | Percent (%) | Number of substitution sites | Substitution rate (%) | 2 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| 6 | 6 | 7 | 7 | 8 | 1 | 1 | 2 | 3 | 3 | 4 | 4 | 4 | 5 | 5 | 6 | 6 | 6 | 6 | 7 | 7 | 8 | 8 | 8 | 8 | 9 | 0 | 0 | 1 | 1 | 3 | 4 | 4 | 4 | 5 | 6 | 6 | 8 | 8 | 8 | 8 | 9 | 9 | 9 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 4 | 4 | 5 | 5 | 6 | 6 | 6 | 6 | 7 | 7 | 7 | 9 | |||||
| 5 | 8 | 8 | 9 | 1 | 1 | 8 | 0 | 8 | 9 | 3 | 6 | 7 | 5 | 8 | 0 | 4 | 6 | 9 | 1 | 8 | 2 | 4 | 5 | 9 | 4 | 0 | 1 | 0 | 7 | 4 | 4 | 7 | 9 | 9 | 2 | 5 | 1 | 3 | 6 | 8 | 0 | 1 | 8 | 1 | 5 | 6 | 7 | 0 | 3 | 4 | 6 | 7 | 0 | 2 | 3 | 1 | 5 | 6 | 7 | 8 | 4 | 6 | 2 | 7 | 3 | 5 | 7 | 8 | 2 | 4 | 6 | 5 | |||||
| I | P | D | A | Q | D | I | E | T | M | M | A | A | G | V | N | L | L | A | I | E | G | R | M | S | H | M | T | A | N | A | N | Q | S | T | I | F | S | I | P | D | T | A | I | N | K | D | A | L | T | N | V | L | T | P | V | S | P | T | V | T | V | L | Q | K | V | L | D | Y | A | S | S | Y | |||||
| 2X 01 | 40 | 13.3 | 0 | 0.0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 2X 02 | 28 | 9.3 | 1 | 0.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | – | – | – |
| 2X 03 | 2 | 0.7 | 1 | 0.3 | – | – | – | S | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 2X 04 | 4 | 1.3 | 1 | 0.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | T | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 2X 05 | 1 | 0.3 | 1 | 0.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | K | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 2X 06 | 1 | 0.3 | 1 | 0.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – |
| 2X 07 | 1 | 0.3 | 1 | 0.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | R | – | – | – | – | – | – | – | – |
| 2X 08 | 14 | 4.7 | 2 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | N | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | – | – | – |
| 2X 09 | 1 | 0.3 | 2 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | T | – | – | – | – | – | – | – | – | – | – | – | – | – | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 2X 10 | 2 | 0.7 | 8 | 2.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | N | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | S | N | N | V | A | N | – |
| 2X 11 | 1 | 0.3 | 8 | 2.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | N | – | – | V | – | – | – | S | N | N | V | A | N | – |
| 2X 12 | 3 | 1.0 | 8 | 2.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | I | – | – | – | – | – | – | – | – | – | – | V | – | – | T | E | N | N | V | – | H | – |
| 2X 13 | 1 | 0.3 | 8 | 2.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | S | N | N | V | A | N | – |
| 2X 14 | 1 | 0.3 | 9 | 2.7 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | G | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | I | – | – | – | – | – | – | - | – | – | – | V | – | – | T | E | N | N | V | – | H | – |
| 2X 15 | 7 | 2.3 | 10 | 3.0 | – | T | – | – | – | – | – | – | – | – | T | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | A | – | – | – | - | – | – | – | V | – | – | – | S | N | N | V | A | N | – |
| 2X 16 | 1 | 0.3 | 10 | 3.0 | – | T | N | – | – | – | L | K | A | – | T | – | – | – | – | – | – | V | V | – | – | – | G | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | N | – | – | – | – | – |
| 2X 17 | 4 | 1.3 | 11 | 3.3 | – | T | N | – | – | – | L | K | A | – | T | – | – | – | – | – | – | – | V | – | – | T | G | – | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | N | – | – | – | – | – |
| 2X 18 | 1 | 0.3 | 14 | 4.1 | – | – | – | – | – | – | – | – | – | – | – | – | – | S | Y | – | – | – | V | – | D | T | – | – | L | L | – | S | T | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | – | – | – | H | – | – | – | – | – | K | – | – | – | – | – | – | – | – | – | N | – | – | – | N | – |
| 2X 19 | 1 | 0.3 | 15 | 4.4 | – | T | N | – | – | – | L | K | – | – | T | – | – | – | – | D | – | – | V | T | – | – | – | – | – | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | H | – | – | – | – | – | – | – | – | – | I | – | – | – | – | – | T | N | H | – | – | N | – |
| 2X 20 | 1 | 0.3 | 16 | 4.7 | – | T | N | – | – | – | L | K | – | – | T | – | – | – | – | – | – | – | – | – | D | T | G | – | L | – | – | S | – | – | S | – | M | A | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | I | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | N | – | – | – | N | – |
| 2X 21 | 1 | 0.3 | 18 | 5.3 | L | – | – | – | L | N | – | – | – | – | T | S | S | S | Y | – | – | – | V | – | D | T | – | – | L | L | – | S | T | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | I | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | N | – | – | – | N | – |
| 2X 22 | 4 | 1.3 | 18 | 5.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | L | – | – | L | – | – | S | V | – | – | – | A | – | S | E | H | – | M | – | – | – | – | – | N | – | N | – | – | V | – | T | E | N | N | V | – | H | – |
| 2X 23 | 1 | 0.3 | 19 | 5.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | M | – | – | L | – | – | L | – | – | S | V | – | – | – | A | – | S | E | H | – | M | – | – | – | – | – | N | – | N | – | – | V | – | T | E | N | N | V | – | H | – |
| 2X 24 | 1 | 0.3 | 19 | 5.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | S | – | M | A | – | – | – | T | L | – | N | – | V | – | – | – | – | – | Q | – | H | – | – | – | – | R | – | A | – | – | N | – | – | V | – | – | S | N | N | V | A | N | – |
| 2X 25 | 1 | 0.3 | 19 | 5.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | S | Y | – | – | – | – | – | D | T | G | – | L | – | – | S | – | – | – | – | M | A | – | L | – | – | – | L | – | – | – | – | – | – | – | – | – | – | – | – | I | – | – | – | – | – | – | – | – | – | – | V | – | – | S | N | N | V | A | N | – |
| 2X 26 | 1 | 0.3 | 25 | 7.4 | – | T | N | – | – | – | L | K | – | – | T | – | – | – | – | – | – | V | – | – | – | – | – | – | L | – | – | – | – | – | – | – | – | – | – | L | – | – | – | T | N | S | V | – | K | E | – | – | – | – | – | I | – | – | – | L | Q | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | – |
| 2X 27 | 2 | 0.7 | 31 | 9.2 | L | – | – | V | – | – | – | K | A | – | T | – | S | – | Y | – | – | – | – | – | A | S | G | T | – | – | – | – | – | S | – | – | M | – | – | L | – | – | L | – | – | S | V | – | – | – | A | – | S | D | Y | – | M | – | – | – | – | – | N | – | N | – | – | V | – | T | E | N | N | V | – | H | – |
| 2X 28 | 32 | 10.7 | 35 | 10.4 | L | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | – | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | K | E | – | T | – | – | – | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | – | T | N | – |
| 2X 29 | 1 | 0.3 | 36 | 10.7 | L | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | – | T | G | – | L | – | – | S | – | K | – | S | – | – | I | L | – | – | – | T | N | S | V | – | K | E | – | T | – | – | – | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | – | T | N | – |
| 2X 30 | 6 | 2.0 | 36 | 10.7 | L | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | – | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | – | T | N | – |
| 2X 31 | 3 | 1.0 | 36 | 10.7 | – | T | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | – | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | – | T | N | – |
| 2X 32 | 1 | 0.3 | 36 | 10.7 | – | T | – | – | L | N | – | K | A | – | T | – | – | S | Y | – | F | – | – | T | A | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | – | T | N | – |
| 2X 33 | 2 | 0.7 | 37 | 10.9 | L | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | – | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | – |
| 2X 34 | 7 | 2.3 | 37 | 10.9 | – | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | D | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | – |
| 2X 35 | 11 | 3.7 | 37 | 10.9 | – | T | N | – | – | – | L | K | A | – | T | S | S | S | Y | – | F | – | – | T | – | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | – | T | N | – |
| 2X 36 | 1 | 0.3 | 38 | 11.2 | – | T | N | – | – | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | – | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | K | E | – | T | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | – | T | N | – |
| 2X 37 | 49 | 16.3 | 38 | 11.2 | L | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | D | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | – |
| 2X 38 | 21 | 7.0 | 38 | 11.2 | L | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | D | T | G | – | L | – | – | S | T | – | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | – |
| 2X 39 | 4 | 1.3 | 38 | 11.2 | L | – | – | – | L | N | – | – | A | F | – | S | S | S | Y | – | F | – | – | T | – | T | G | – | – | – | T | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | F |
| 2X 40 | 1 | 0.3 | 38 | 11.2 | – | – | – | – | – | N | – | – | A | F | – | S | S | S | Y | – | F | – | – | T | A | T | G | – | L | – | T | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | F |
| 2X 41 | 33 | 11.0 | 40 | 11.8 | L | – | – | – | L | N | – | – | A | F | – | S | S | S | Y | – | F | – | – | T | A | T | G | – | L | – | T | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | F |
| 2X 42 | 1 | 0.3 | 42 | 12.4 | L | – | – | – | L | N | – | – | A | F | – | S | S | S | Y | – | F | M | V | T | G | T | G | – | L | – | T | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | – | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | F |
| 2X 43 | 1 | 0.3 | 39 | 11.5 | L | – | – | – | L | N | – | – | A | – | T | S | S | S | Y | – | F | – | – | T | D | T | G | – | L | – | – | S | – | K | – | S | – | – | – | L | – | – | – | T | N | S | V | – | – | – | E | V | T | N | H | I | – | – | – | L | Y | – | I | I | – | – | V | – | – | – | S | N | – | V | T | N | – |