TABLE 9.
Deduced amino acid sequences of PBP1a in 300 S. pneumoniae isolates.
| PBP1a group | n | Percent (%) | Number of substitution sites | Substitution rate (%) | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
| 1 | 1 | 1 | 1 | 2 | 2 | 2 | 3 | 3 | 4 | 5 | 5 | 7 | 8 | 8 | 8 | 8 | 9 | 9 | 9 | 9 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 7 | 7 | 9 | 9 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 3 | 4 | 4 | 4 | 5 | 5 | 6 | 6 | 7 | 7 | 7 | 7 | 7 | 7 | 8 | 8 | 9 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 3 | 3 | 4 | 4 | 4 | 4 | 4 | 5 | 5 | |||||
| 2 | 6 | 7 | 8 | 0 | 1 | 6 | 3 | 7 | 7 | 1 | 8 | 1 | 2 | 5 | 6 | 8 | 2 | 3 | 5 | 7 | 5 | 6 | 8 | 3 | 4 | 1 | 2 | 3 | 9 | 2 | 3 | 4 | 5 | 5 | 7 | 3 | 5 | 2 | 4 | 5 | 6 | 7 | 8 | 0 | 3 | 0 | 3 | 6 | 0 | 3 | 6 | 8 | 0 | 1 | 4 | 5 | 6 | 7 | 3 | 5 | 2 | 6 | 7 | 9 | 1 | 2 | 5 | 6 | 2 | 3 | 8 | 9 | 0 | 2 | 5 | 6 | 8 | 0 | 1 | 3 | 5 | 9 | 0 | 4 | |||||
| D | T | D | E | V | A | E | I | S | A | S | I | T | L | G | V | E | T | I | H | E | N | T | V | R | G | L | P | N | I | S | D | K | K | T | Y | H | V | E | E | F | S | N | V | T | D | S | T | N | A | P | E | I | N | H | T | S | Q | F | L | A | S | L | V | N | L | T | A | K | M | T | G | S | N | E | N | I | E | L | Y | N | E | K | N | S | |||||
| 1A 01 | 40 | 13.3 | 1 | 0.3 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | - | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 02 | 5 | 1.7 | 2 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | R | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 03 | 5 | 1.7 | 2 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | Q | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | - | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 04 | 44 | 14.7 | 2 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | T | – | – | – | – | – | – | – | – | – | – | – | - | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 05 | 11 | 3.7 | 2 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | I | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | - | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 06 | 8 | 2.7 | 2 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | E | – | – | – | – | – | – | – | – | – | – | – | – | - | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 07 | 6 | 2.0 | 3 | 0.9 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | I | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | T | – | – | – | – | – | – | – | – | – | – | – | - | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 08 | 1 | 0.3 | 10 | 2.9 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | D | – | – | – | – | – | – | – | L | – | – | – | – | – | – | – | – | – | – | L | – | – | – | – | – | – | T | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | I | – | – | Q | Q | – | P |
| 1A 09 | 1 | 0.3 | 15 | 4.4 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | I | G | P | – | – | – | K | Y | N | T | G | Y | M | V | A | – | – | D | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 10 | 1 | 0.3 | 18 | 5.2 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | D | – | – | – | – | D | – | – | – | – | – | – | – | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | V | I | G | P | – | – | – | K | Y | N | T | G | Y | M | V | A | – | – | D | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | – | – | – | – | – |
| 1A 11 | 2 | 0.7 | 25 | 7.3 | N | – | – | – | – | – | – | – | T | – | – | – | – | – | – | – | D | – | – | – | – | D | I | – | – | – | – | – | – | – | – | N | – | – | I | H | N | I | – | – | – | – | D | A | – | E | V | I | G | P | – | – | – | K | Y | N | T | G | Y | – | – | – | – | – | D | – | – | – | – | – | – | – | – | – | – | – | V | – | – | – | – | – | – | – | – |
| 1A 12 | 1 | 0.3 | 27 | 7.8 | N | – | – | – | – | – | – | – | T | – | – | – | S | – | – | – | D | – | – | – | – | D | I | – | – | – | – | – | – | L | – | – | Q | – | – | – | – | – | – | – | – | – | – | – | – | – | T | – | G | P | – | – | – | K | Y | N | T | G | Y | M | V | – | I | I | D | F | L | – | – | – | – | – | – | – | – | – | V | D | I | – | – | – | – | – | – |
| 1A 13 | 1 | 0.3 | 36 | 10.5 | – | S | – | – | – | S | D | V | – | – | A | T | A | I | – | – | D | – | M | N | I | S | – | – | – | A | – | T | D | M | A | N | – | Q | – | – | – | – | K | – | – | – | – | – | – | – | T | – | G | P | – | – | V | – | – | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 14 | 1 | 0.3 | 37 | 10.8 | – | S | – | – | – | S | D | V | – | – | A | T | S | – | – | – | D | S | – | – | V | D | – | L | H | V | I | T | D | M | A | N | – | Q | – | – | – | – | K | – | – | – | – | – | – | – | T | – | G | P | – | – | V | – | – | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 15 | 86 | 28.7 | 43 | 12.5 | – | S | – | – | – | S | D | V | – | – | A | T | S | – | – | – | D | S | – | – | V | D | – | L | H | V | I | T | D | M | A | N | – | – | I | H | N | I | – | – | – | – | D | A | – | E | T | – | G | P | – | – | – | K | Y | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 16 | 9 | 3.0 | 43 | 12.5 | – | S | – | – | – | S | D | V | – | – | A | T | S | – | – | – | D | – | M | N | I | S | – | – | – | A | – | T | D | M | A | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | A | – | G | P | – | D | – | K | – | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | I | S | D | D | Q | G | T | M | D | – | F | – | – | – | – | – |
| 1A 17 | 3 | 1.0 | 44 | 12.8 | – | S | – | – | – | S | D | V | – | – | A | T | S | – | – | – | D | S | – | – | V | D | – | L | H | V | I | T | D | M | A | N | – | – | I | H | N | I | – | – | – | – | D | A | I | E | T | – | G | P | – | – | – | K | Y | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 1A 18 | 35 | 11.7 | 45 | 13.1 | – | S | – | – | – | S | D | V | – | – | A | T | A | I | – | – | D | – | M | N | I | S | – | – | – | A | – | T | D | M | A | N | – | Q | – | – | – | – | K | – | – | – | – | – | – | – | T | – | G | P | – | – | V | – | – | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | I | – | D | T | H | – | T | M | D | – | F | – | – | – | – | P |
| 1A 19 | 9 | 3.0 | 46 | 13.4 | – | – | – | – | – | – | – | V | – | – | A | T | A | I | – | – | D | – | M | N | I | S | – | – | – | A | – | T | D | M | A | N | – | Q | I | H | N | – | – | – | – | – | D | – | – | E | A | – | G | P | A | D | – | K | – | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | I | – | D | T | H | – | T | M | D | – | F | – | – | – | – | – |
| 1A 20 | 2 | 0.7 | 49 | 14.2 | – | – | – | – | – | – | – | – | – | S | – | – | A | – | D | I | D | S | – | – | V | D | – | L | K | V | I | T | D | M | A | N | – | – | I | H | N | I | S | – | Y | A | D | P | – | E | A | – | G | P | – | D | – | – | Y | N | T | G | Y | M | V | – | I | – | D | F | Y | – | – | – | – | D | N | – | G | T | M | – | – | – | S | – | – | K | – |
| 1A 21 | 1 | 0.3 | 50 | 14.5 | – | – | – | – | I | – | – | – | – | S | – | – | A | – | D | I | D | S | – | – | V | D | – | L | K | V | I | T | D | M | A | N | – | – | I | H | N | I | S | – | Y | A | D | P | – | E | A | – | G | P | – | D | – | – | Y | N | T | G | Y | M | V | – | I | – | D | F | Y | – | – | – | – | D | N | – | G | T | M | – | – | – | S | – | – | K | – |
| 1A 22 | 5 | 1.7 | 51 | 14.8 | – | S | – | – | – | S | D | V | – | – | A | T | A | I | – | – | D | – | M | N | I | S | – | – | – | A | – | T | D | M | A | N | – | Q | I | H | N | – | K | – | – | – | D | – | – | E | A | – | G | P | A | D | – | K | – | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | I | – | D | T | H | – | T | M | D | – | F | – | – | – | – | – |
| 1A 23 | 1 | 0.3 | 52 | 15.1 | – | S | E | – | – | S | D | V | – | – | A | T | – | I | – | – | D | – | M | N | I | S | – | – | – | A | – | T | D | – | A | N | – | H | I | H | N | I | – | – | – | – | D | A | – | E | A | – | G | P | – | D | – | K | – | N | T | G | Y | M | V | – | I | – | D | F | L | – | – | I | S | D | D | Q | G | T | M | D | – | F | – | – | – | – | – |
| 1A 24 | 21 | 7.0 | 53 | 15.4 | – | S | – | – | – | S | D | V | – | – | A | T | S | – | – | – | D | S | – | – | V | D | – | L | H | V | I | T | D | M | A | N | – | – | I | H | N | I | – | – | – | – | D | A | – | E | T | – | G | P | – | – | – | K | Y | N | T | G | Y | M | V | – | I | I | D | F | L | G | R | – | – | D | N | H | G | T | M | – | – | – | S | – | – | – | – |
| 1A 25 | 1 | 0.3 | 55 | 16.0 | – | S | – | – | – | S | D | V | – | – | A | T | S | – | – | – | D | S | – | – | V | D | – | L | H | V | I | T | D | M | A | N | – | – | I | H | N | I | – | – | – | – | D | A | – | E | T | – | G | P | – | – | – | K | Y | N | T | G | Y | M | V | – | I | I | D | F | L | G | R | – | – | D | N | H | G | T | M | – | – | – | S | – | Q | – | N |