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. 2020 Mar 30;14(10):1462–1472. doi: 10.1093/ecco-jcc/jjaa065

Figure 4.

Figure 4.

Reg3B modulates DSS colitis. [A] Body weight changes [left] and histological scores [middle] of inflamed gut epithelium during/after a 2% DSS treatment of Reg3B-KO mice vs controls; right, per cent Ki-67+ cells per total cells per colon crypt in WT vs Reg3B-KO mice. Representative DSS day-8 images of colon sections stained with haematoxylin and eosin. [B] Body weight changes [left], histological scores [middle] and Ki-67 staining of colon crypts [right] in WT mice treated with propionate [50 mM] vs control and 3% DSS for another 5 days; propionate was administered 2 days before DSS until tissue harvest on day 8 or 14. [C] Expression of Lgr5 [left] and Olfm4 [right] in small intestinal organoids after adding recombinant murine Reg3B [100 µM] and/or 0.01% DSS to culture media; photomicrographs of non-treated [CTR] and DSS-treated [DSS] organoids. [D] mRNA expression in organoids of Lgr5, Olfm4 or clusterin after adding propionate [PROP, 1 mM] and/or 0.01% DSS to the culture media. Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. SI organoids: n = 7–10 biological replicates per group; mouse experiments: n = 7–12 mice per group.