TABLE 1.
Gene | Primer sequences (5’‐3’) | Product size (bp) | Temp (°C) |
---|---|---|---|
NCL | F: CCACTTGTCCGCTTCACAC | 352 | 55 |
R: ACCAGGAGTTGCTACCAATG | |||
PIK3R1 | F: TGTCAGTGATTGAAGAGCAT | 348 | 55 |
R: TACAGTCCAGAAGTTCCATAG | |||
AKT | F: GTATCTCAGTCTAAGGTCTCAT | 448 | 60 |
R: GGCTTCTTCTACAGTATCCA | |||
GNB4 | F: TGGTGCTTGTGATGCCTCTT | 378 | 55 |
R: ACAGCCATGCCATCATCAGT | |||
CDK6 | F: GTGAACCAGCCCAAGATGAC | 116 | 60 |
R: TGGAGGAAGATGGAGAGCAC | |||
IL6R | F: ACCAGACAGGTGCGAAAG | 215 | 50 |
R: CTCCCAAAGTGCTAGGATTA | |||
LAMA1 | F: CTGACGGGTTCTATGGG | 368 | 54 |
R: GTCGCAGGTATTCTGAGTG | |||
PDGFD | F: AAGGAAACGGCTACG | 290 | 50 |
R: GTCATCGGACTTGAATG | |||
PPP2R3A | F: GCATTCCGACCTTCTACTT | 292 | 55 |
R: AGCAGATGGACGAACTTG | |||
FGF2 | F: CCATTGTCCCAGTAAA | 448 | 50 |
R: CACCAGCAGAGTTGC | |||
ITGA5 | F: GGGAGGTTTAGGAAGCG | 251 | 58 |
R: CCACTGAGAATCCGAAGAA | |||
PRKCA | F: TAGCACCGTCCGAATC | 331 | 50 |
R: TGAAAGGAAGGGAAGCA | |||
TNXB | F: TGGGAAGGCTACGTGAGTG | 340 | 58 |
R: CTGGGCATGTCTGGATGG | |||
VEGF | F: CTTGCCTTGCTGCTCTAC | 278 | 58 |
R: ATGGTGATGTTGGACTCCT | |||
β‐actin | F: CTACAATGAGCTGCGTGTGG | 528 | 58 |
R: AAGGAAGGCTGGAAGAGTGC |
Abbreviation: NCL, nucleolin.