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. 2022 Feb 6;11(6):1454–1464. doi: 10.1002/cam4.4569

TABLE 1.

Primer sequences and product sizes

Gene Primer sequences (5’‐3’) Product size (bp) Temp (°C)
NCL F: CCACTTGTCCGCTTCACAC 352 55
R: ACCAGGAGTTGCTACCAATG
PIK3R1 F: TGTCAGTGATTGAAGAGCAT 348 55
R: TACAGTCCAGAAGTTCCATAG
AKT F: GTATCTCAGTCTAAGGTCTCAT 448 60
R: GGCTTCTTCTACAGTATCCA
GNB4 F: TGGTGCTTGTGATGCCTCTT 378 55
R: ACAGCCATGCCATCATCAGT
CDK6 F: GTGAACCAGCCCAAGATGAC 116 60
R: TGGAGGAAGATGGAGAGCAC
IL6R F: ACCAGACAGGTGCGAAAG 215 50
R: CTCCCAAAGTGCTAGGATTA
LAMA1 F: CTGACGGGTTCTATGGG 368 54
R: GTCGCAGGTATTCTGAGTG
PDGFD F: AAGGAAACGGCTACG 290 50
R: GTCATCGGACTTGAATG
PPP2R3A F: GCATTCCGACCTTCTACTT 292 55
R: AGCAGATGGACGAACTTG
FGF2 F: CCATTGTCCCAGTAAA 448 50
R: CACCAGCAGAGTTGC
ITGA5 F: GGGAGGTTTAGGAAGCG 251 58
R: CCACTGAGAATCCGAAGAA
PRKCA F: TAGCACCGTCCGAATC 331 50
R: TGAAAGGAAGGGAAGCA
TNXB F: TGGGAAGGCTACGTGAGTG 340 58
R: CTGGGCATGTCTGGATGG
VEGF F: CTTGCCTTGCTGCTCTAC 278 58
R: ATGGTGATGTTGGACTCCT
β‐actin F: CTACAATGAGCTGCGTGTGG 528 58
R: AAGGAAGGCTGGAAGAGTGC

Abbreviation: NCL, nucleolin.