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. 2022 Mar 1;12:842418. doi: 10.3389/fonc.2022.842418

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Copyright © 2022 Schneider, Winkler, Kell, Pfaffl, Atkinson and Moertl

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enhanced survival of irradiated HNSCC cells after overexpression of GRP78. (A–C) Western Blot analysis of GRP78 shows increased expression in BHY (A) and FaDu (B) cells 24 h, 48 h and 72 h after transfection with GRP78 plasmid (pGRP78). GRP78 expression was normalized to beta-actin and control transfection (pcDNA) served as baseline (n = 4, 2-way RM ANOVA, *p adj. < 0.05). (C) Representative Western Blot images of GRP78 expression in pGRP78- and pcDNA-transfected BHY and FaDu cells. Beta-actin was used as loading control. (D, E) Clonogenic survival assay of BHY (D) and FaDu (E) cells exposed to radiation doses of 0, 2, 4 or 8 Gy 72 h after GRP78 transfection. Both cell lines show increased survival in GRP78 overexpressing cells. Values were normalized to 0 Gy control. Logarithmic scale was used for the relative cell survival (n = 4, Two-way RM ANOVA).