Figure 7.

Increased GRP78 expression in HNSCC EV recipient cells. (A, B) Western Blot analysis with representative blot (B) of GRP78 expression in EV recipient cells show increased amounts of GRP78 in 6Gy-EV recipient cells. GRP78 expression was normalized to beta-actin and 0Gy-EV recipient cell GRP78 expression served as baseline (n = 3, t-test, *p < 0.05). (C) Fluorescence microscopy images of BHY cells co-cultured with PKH67-stained EVs documenting uptake. Stained vesicle membranes are shown in green. Recipient cell nuclei are shown in blue. White size bar indicates 20 µm.