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. 2020 Jun 30;225(6):1040–1049. doi: 10.1093/infdis/jiaa375

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© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Gating strategy to characterize microvesicles (MVs) by flow cytometry. (a) Microvesicles less than 1 μM were gated using size reference beads and CD9 expression. Representative contour plots showing side scatter (SSC) and forward scatter (FSC) or CD9 labeling of MVs from antiretroviral therapy-exposed human immunodeficiency virus-infected donors (n = 30); (b) CD3 staining on plasma-derived MVs or peripheral blood-derived T cells; CD14 staining on plasma-derived MVs or monocytes, and CD41a and CD61 staining on plasma-derived MVs or platelets; (c) coexpression of CD11b and CD16 on MVs and CD66b staining on plasma-derived MVs subsets or peripheral blood-derived neutrophils.