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. 2022 Jan 22;9(8):2104472. doi: 10.1002/advs.202104472

Figure 4.

Figure 4

Preparation, characterization, and the cellular uptake of JX06‐NPs. A) Preparation of JX06‐NPs. B,C) Characterization of JX06‐NPs by TEM and DLS. Scale bar: 100 nm. D,E) The intracellular uptake of JX06‐NPs by IshikawaHG and IshikawaNG at various time points from 0 to 7 h detected by flow cytometry and CLSM. JX06‐NPs were labeled by Cy5.5. Scale bar: 20 µm. Unpaired Student's t‐test. F) 3D cell spheres of IshikawaHG treated with JX06‐NPs were observed by CLSM from different sections (5–30 µm). Scale bar: 100 µm. Data are shown as mean ± SD. **P < 0.01.