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. 2022 Jan 5;41(6):e108946. doi: 10.15252/embj.2021108946

Figure EV2. CDK4/6i induces cellular senescence dependent on p53.

Figure EV2

  1. Immunoblot of p53 on cells of indicated genotypes and treatments.
  2. Representative images of p53GSE BJ cells at the end of the 8‐day indicated treatments (scale bar, 1 mm; n = 3 independent experiments).
  3. 8‐day population doubling of treated p53GSE BJ cells (n = 3 independent experiments).
  4. 3 × 103 vehicle or palbociclib‐treated (1 μM for 8 times in 24 h) scramble/shp53/ p53GSE BJ cells were replated in 6‐well dishes, cultured for 8 days, and stained with 0.2% crystal violet (n = 3 independent experiments).
  5. 3 × 103 vehicle‐ or abemaciclib‐treated (4 μM for 8 times in 24 h) WT/p53GSE MEFs were replated in 6‐well dishes, cultured for 8 days and stained with 0.2% crystal violet (n = 3 independent experiments).
  6. BJ cells were treated with abemaciclib ± pifithrin‐α and EdU staining performed at 8 dpt (n = 9 samples from 3 independent experiments).

Data information: Data are means ± SD. One‐way ANOVA (C). Unpaired two‐tailed t‐test (F). ***P < 0.001.