Figure 7. Constitutive palbociclib treatment in various tumour lines causes cell cycle delays and DNA damage.

• A
  Quantification of cumulative mitotic entry over time immediately following 1 μM palbociclib treatment in a range of tumour cell lines, as indicated. Mitotic entry of 50 cells per condition per experiment were analysed immediately following drug addition. Graph shows cumulative mean ± SD from three experiments (150 cells total).
• B, C
  Quantification of the nuclear morphologies (B) or γH2AX‐positive DNA damage foci (C) from different cancer lines, as indicated, treated continuously with palbociclib (1 μM) for 0, 1, 2 or 3 weeks. Either 100 cells (nuclear morphology) or 50 cells (γH2AX) were scored per condition per experiment and bar graphs represent mean data + SEM from three experiments.
• D
  Quantification of weekly proliferation rate in cells treated as in (B). A total of 60,000 cells of each cell line were cultured in 10 cm dishes with 1 μM palbociclib for a total of 3 weeks. Every 7 days, cells were trypsinized and total cell counts were determined. The 0‐week time point shows the 7‐day fold increase of untreated cells. The data points show fold increase in cell count over each 7‐day period, and bars represent the mean from two experiments.