Table 1. Cryo-EM Data Collection, Refinement, and Validation Statistics.
| data collection and processing | model comparison | ||
|---|---|---|---|
| magnification | ×105 000 | nonhydrogen atoms | 8990 |
| voltage (kV) | 300 | protein residues | 1070 |
| electron exposure e-/Å2 | 54 | B factors (Å2) | |
| defocus range (μm) | 0.8–2.0 | protein | 21–306 |
| pixel size (Å) | 0.859 | r.m.s deviations | |
| symmetry imposed | C1 | bond lengths (Å2) | 0.0126 |
| initial particle images (no) | 2000000 | bond angles (°) | 1.1569 |
| final particle images (no) | 196709 | validation | |
| map resolution (Å) | 2.8 | MolProbity score | 1.47 |
| FSC threshold | 0.143 | clashscore | 4.22 |
| map resolution range (Å) | 2.8 to > 5.5 | poor rotamers (%) | 1.28 |
| refinement | Ramachandran plot | ||
|---|---|---|---|
| initial model used | 5LEW | favored (%) | 96.90 |
| model resolution (Å) | 2.9 | allowed (%) | 3.10 |
| FSC threshold | 0.143 | disallowed (%) | 0 |
| map sharpening B-factor (Å2) | –50 |