TABLE 1.

Experimental conditions for kinetic studies

Antibiotic	Concn range (μM)	Δɛ (M−1 cm−1)	λ (nm)	Enzyme (μg)
Benzylpenicillin	500–1,000	−775	235	0.5–1
Ampicillin	250–1,000	−820	235	1–2
Carbenicillin	800–1,500	−780	235	1–2
Piperacillin	500–2,000	−820	235	1–10
Temocillin	100–1,200	−660	235	5–10
Ticarcillin	100–1,200	−660	235	<10
Imipenem	50–120	−9,000	300	0.02–0.05
Meropenem	100–200	−6,500	300	0.5–2
Panipenem	50–100	−7,400	300	0.02–0.5
Biapenem	100–200	−7,600	293	1–2
Nitrocefin	50–150	+15,000	482	0.05–0.5
Cephaloridine	25–150	−10,000	260	0.5–1
Cephalothin	25–150	−6,500	260	0.5–1
Cefuroxime	50–150	−7,600	260	0.5–1
Ceftazidime	50–120	−9,000	260	5–10
Cefotaxime	50–100	−7,500	260	1–5
Desacetyl-cefotaxime	50–100	−7,250	260	0.05–1
Cefoxitin	25–100	−7,700	260	0.2–0.5
Cefpirome	50–100	−4,500	260	0.5–2
Cefepime	25–75	−10,000	260	1–3
Loracarbef	50–100	−10,600	260	0.05–0.5
Moxalactam	50–100	−4,000	260	0.5–1
Aztreonam	1,000	−700	320	5
Carumonam	1,000	−810	310	5