FIG. 1.

Time course of IL-8 release by human eosinophils. Eosinophils (2.5 × 10⁵/ml, 0.5 ml) were incubated with or without 10⁻⁶ M calcium ionophore in a humidified atmosphere at 37°C with 5% CO₂. IL-8 release was expressed as a percentage of the spontaneous IL-8 release in 24 h, which was defined as 100%. There was a spontaneous release of IL-8 which was inhibited by the addition of 10-μg/ml cycloheximide. Each point represents the mean ± the SEM of four separate experiments. ∗, P < 0.001 compared to the spontaneous group at each time point.