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. Author manuscript; available in PMC: 2022 Mar 15.
Published in final edited form as: Matrix Biol. 2020 Aug 7;94:31–56. doi: 10.1016/j.matbio.2020.07.002

Fig. 8.

Fig. 8.

(A, B) Periostin expression in mPFBs is regulated by matrix stiffness: WT mPFBs were cultured on silicone substrates of different stiffness, with or without TGFβ, for (A) 24- and (B) 72-hours, and Postn mRNA levels were quantified using RTqPCR. Values are given as mean±SD from 3 independent experiments. Data was analyzed using ANOVA, (*P < 0.05; **P < 0.005). (C) Matrix stiffness alone is not sufficient to restore the contractile phenotype of Postn−/− cells: mPFBs were cultured on silicone substrates of different stiffness and analysed for α-SMA incorporation in stress fibers. (D) Quantification of α-SMA positive stress fiber-cells. (E) mPFBs were treated with 5 ng/ml TGFβ and α-SMA positive stress fiber-cells were quantified using immunofluorescence. (F) Acta2 expression was quantified RTqPCR. (G) Western blot was used to assess α-SMA protein level of WT and Postn−/− mPFBs. GAPDH was used as a loading control. Values are given as mean±SD from 3 independent experiments. Data was analyzed using ANOVA, (*P < 0.05; **P < 0.005). Scale bar: 20 µm.