Table 1.
Application of CRISPR/Cas based gene editing in HIV-1/AIDS therapy.
| CRISPR-Cas system | Delivery | Target | Cell type/organism | Animal model | AIDS patient | Ref |
|---|---|---|---|---|---|---|
| SpCas9 | Transfection, Lentivirus, Adenovirus, Electroporation | LTR, Rev, Gag, Pol, Env, CCR5, CXCR4, Restriction factors, | HEK293T, 293T-CD4-CCR5, 293 Primary T cells, Hela, TZM-b1, Jurkat, SupT1, K562, hPSC, iPSC, CHO, human CD34+ HSPCs, CHME5, U937, JLat10.6, J.Lat FL, CEMss, C8166, Ghost, pMoHIV |
Mouse | Beijing patient NCT 03164135 | (Ebina et al., 2013); (Hu et al., 2014); (Liao et al., 2015); (Wang et al., 2014); (Wang et al., 2016); (Lebbink et al., 2017); (Cho et al., 2013); (Ye et al., 2014); (Li et al., 2015); (Kang et al., 2015); (Xu et al., 2017); (Qi et al., 2018); (Hou et al., 2015); (Z Liu et al., 2017); (Bogerd et al., 2015); (Rosa et al., 2015); (Gonzalez-Enriquez et al., 2017); (Chougui et al., 2018); (Lahaye et al., 2018); (Yurkovetskiy et al., 2018); (Hultquist et al., 2016); (Xu et al., 2019); (Scott et al., 2019); (Sessions et al., 2020); |
| SaCas9 | Lentivirus, AAV, Transfection | LTR, Gag, proviral SIV DNA, CCR5, CXCR4, | HEK293T, TZM-bl, C11, Ghost, Jurkat, primary CD4+ T, human CD34+ HSPCs, J-Lat 6.3 | Tg26 transgenic mouse, SIV-infected macaques | No | (Yin et al., 2017); (Wang et al., 2018); (Wang et al., 2017); (Xiao et al., 2019a); (Olson et al., 2020); (Mancuso et al., 2020) |
| CRISPRa | Lentivirus, Transfection, Electroporation | LTR, APOBEC3G, APOBEC3B, T5, T6, | TZM-b1, J-Lat, CHME5 microglial cell, C11, HEK293T, ACH2, J1.1, CEM LChIT 3.2, Hela, U1 | No | No | (Zhang et al., 2015); (Saayman et al., 2016); (Limsirichai et al., 2016); (Ji et al., 2016); (Bogerd et al., 2015); (Kim et al., 2017); (Zhang et al., 2018) |
| CRISPRi | Lentivirus | PSMD 1, PSMD 3, PSMD 8, Spt4, Spt5, host factors (FTSJ3, TMEM178A, NICN1) | Jurkat 2D10 | No | No | (Z Li et al., 2019); (Krasnopolsky et al., 2021); (Z Li et al., 2020) |
| CPF1 | Adenovirus, Lentivirus | CCR5, LTR, Gag, Vpr, Tat, Rev | TZM-b1, SupT1-R5, Primary CD4+T cells, HEK293T | No | No | (Liu et al., 2020); (Gao et al., 2020) |
| Base editor | No | No | No | No | No | No |
| Prime editing | No | No | No | No | No | No |
| SHERLOCK | No | No | No | No | No | No |
| DETEECTR | No | No | No | No | No | No |
| PAC-MAN | No | No | No | No | No | No |
| ABACAS | No | No | No | No | No | No |