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. 2022 Jan 21;37(1):115–126. doi: 10.1016/j.virs.2022.01.021

Fig. 4.

Fig. 4

Increased virulence of ZIKV in association with specific mutations on E protein but not on NS2A protein. A Scheme of mutation strategy based on pFLZIKV (CAM-WT) infectious clone to create CAM-M1 (CM1), CAM-M2 (CM2), CAM-M3 (CM3) viruses; B IFA of ZIKV E protein expression at indicated times (24, 48, 72 ​h post transfection) in BHK-21 ​cells transfected with RNA from CAM- WT or mutant viruses (CM1, CM2, CM3). C Survival curve of DP2 BALB/c mice infected i.c. with 10 ​PFU CAM-WT, mutant viruses (CM1, CM2, CM3), or PBS (n ​= ​10 for each group). D–H. DP2 BALB/c mice were infected s.c. with 100 ​PFU CAM-WT, mutant viruses, or PBS (n ​= ​8 for each group). D Survival was monitored and analyzed from 0 to 25 days post infection; E–F. Body weight difference between PBS-Ctl (PBS), CAM-WT (WT) and CAM-M1 (CM1) groups at 3- and 11-days post infection (3 dpi and 11 dpi); G–H The morbidity of CAM-WT and CAM-M1 groups (clinical score: 0-healthy, 1-manic and limb weakness, 2-limb paralysis, 3-moribund or death); the summary data were presented as mean ​± ​standard deviation (SD) and analyzed by student’s t-test; Survival rate was analyzed by log rank test; P values were indicated by ∗ (P ​< ​0.05), or ∗∗ (P ​< ​0.01), or ∗∗∗ (P ​< ​0.001). Data shown are representative of two independent experiments.