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. 2022 Jan 21;37(1):115–126. doi: 10.1016/j.virs.2022.01.021

Fig. 6.

Fig. 6

Molecularly cloned ZIKV with a D67N single mutation on E protein has marked increase in the infection of brain tissues. A DP2 BALB/c mice were infected s.c. with 100 ​PFU CAM-WT or CM1 (n ​= ​8 for each group). Virus RNA loads in tissues (brain, blood, spleen, liver and kidney) at 3 days post infection (3 dpi) and 11 days post infection (11 dpi) were determined by real-time PCR; B–C DP2 BALB/c mice were infected i.c. with 20 ​PFU CAM-WT, CM1 and CM1-A (n ​= ​5–7 for each group). Virus RNA load in brains at 3 dpi (B) and 11 dpi (C) were determined by real-time PCR; D DP2 BALB/c mice were infected s.c. with 100 ​PFU CAM-WT or CM1-A (n ​= ​6 for each group). Virus RNA loads in brains at 3 dpi and 11 dpi were determined by real time PCR; The summary data were presented as mean ​± ​standard deviation (SD) and analyzed by student’s t-test; P values were indicated by ∗ (P ​< ​0.05), or ∗∗ (P ​< ​0.01), or ∗∗∗ (P ​< ​0.001). Data shown are representative of two independent experiments.