Table 1.
Cryo-EM data collection, refinement and model validation statistics.
| Item | CCHFV sGc-trimer |
|---|---|
| Data collection and processing | |
| Microscope | JEOL CRYO ARM 300 |
| Voltage (kV) | 300 |
| Magnification | 80,000 × |
| Defocus range (μm) | 0.5 to 2.5 |
| Pixel size (Å) | 0.61 |
| Image stacks (no.) | 3330 |
| Frames per stack | 40 |
| Total dose per movie (e−/Å2) | 48 |
| Final particle images (no.) | 187,292 |
| Symmetry imposed | C3 |
| Map resolution (Å) | 2.8 |
| FSC threshold | 0.143 |
| Map-sharpening B factor (Å2) | −141 |
| Model refinement | |
| Composition | |
| Chains | 3 |
| Non-hydrogen atoms | 10,143 |
| Protein residues | 1296 |
| B factors for protein (Å2) | 43.91 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 0.765 |
| Validation | |
| MolProbity score | 1.87 |
| Clashscore | 7.79 |
| Poor rotamers (%) | 0.00 |
| Ramachandran plot | |
| Favored (%) | 93.06 |
| Allowed (%) | 6.94 |
| Disallowed (%) | 0.00 |
CCHFV, Crimean-Congo hemorrhagic fever virus; sGc-trimer, trimer of the soluble Gc ectodomain; R.m.s, root mean square.