Fig. 4. Increased fluoroquinolone penetration to primary granulomas after treatment with lactoferrin.

Lungs from Mtb infected mice alone (left), or rHLF treated prophylactically (P; middle) or therapeutically (T; right) were assessed for presence of ofloxacin within granulomatous inflammation. Ofloxacin penetration was primarily excluded from inflammatory foci in the Mtb infected alone group, while both rHLF treated mice permitted entry of ofloxacin into regions of pathology. Top panels represent hematoxylin & eosin (H&E) brightfield stained histographs (40× magnification) with matching fluorescence captured using multispectral imaging (bottom). Example populations of macrophage-like cells (white arrows) and lymphocytic cells (black arrows) are indicated.