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. 2022 Mar 1;12:805290. doi: 10.3389/fonc.2022.805290

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Copyright © 2022 Zhang, Kong, Zhu, Sun, Han, Yan, Cui, Zhang, Zhang, Kang, Dai, Qian and Yan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RAI2 expression is negatively correlated with that of CtBP2. (A) Upper panel: A schematic of RAI2 domain organization and the mutant sites of mutant type RAI2 (RAI2-M); lower panel: Coimmunoprecipitation (Co-IP) analysis of RAI2 and CtBP2 interaction by anti-RAI2 antibody using cell lysates from LoVo cells transfected with empty vector, RAI2 expression vector or RAI2 mutant vector. And Cell lysates from SW620 cells were also used for Co-IP detection to analyze the endogenous interaction between CtBP2 and RAI2 by RAI2 antibody, with IgG group as a negative control. Input: before any antibodies were added. (B) The reciprocal mRNA expression between CtBP2 and RAI2 in 434 cases of colorectal cancer tissues from the TCGA dataset. (C) RT-PCR detection of RAI2 and CtBP2 expression in LoVo/HCT116 cells with or without RAI2 re-expressed and in SW620 cells with or without RAI2 knocked down.