Figure 8.

MSCs induced differentiation of monocytic MDSCs in BM.

A. In C57BL/6 mice, human BM MSCs were injected via the tail vein after EAU induction via IRBP immunization. Examination at days 1 and 7 revealed that the MSCs increased a distinct subset of CD11b^midLy6C^midLy6G^lo cells in BM while reducing the number of CD11b^hiLy6C^hiLy6G^lo cells.

B. In culture, BM cells were extracted from C57BL/6 mice and cocultured with human BM MSCs in a direct coculture or transwell system under GM-CSF stimulation for 5 days. Both direct and transwell cocultures with MSCs induced a distinct subset of CD11b^midLy6C^midLy6G^lo cells, while most of Ly6G^lo BM cells differentiated into CD11b^hiLy6C^hiLy6G^lo cells without MSCs.

Reprinted with permission from JCI Insight. Lee et al., 2020. Mesenchymal stromal cells induce distinct myeloid-derived suppressor cells in inflammation. JCI Insight 5, e136059. Copyright © 2020 American Society for Clinical Investigation.