Table 4.

Performance characteristics of mn-PCR, LM, and LAMP by malaria case in comparison to the gold standard (nPCR) in Sistan-Baluchestan, Iran

Test	Sensitivity% (95% CI)	Specificity% (95% CI)	PPV% (95% CI)	NPV% (95% CI)	Agreement between tests, kappa
All positive by nPCR vs. all positive by LM	92.31 (81.46–97.86)	81.13 (68.03–90.56)	82.76 (73.20–89.40)	91.49 (80.6–96.53)	0.734
All positive by nPCR vs. all positive by mn-PCR	100 (92.45–100)	100 (91.59–100)	100 (0.9–100)	100 (0.89–100)	1
All positive by nPCR vs. all positive by LAMP	92.06 (82.44–97.37)	100 (91.59–100)	100 (0.92–100)	89.36 (78.37–95.12)	0.903

CI = confidence interval; LAMP = loop-mediated isothermal amplification; LM = light microscopy; mn-PCR = multiplex/nested polymerase chain reaction; nPCR = nested polymerase chain reaction; NPV = negative predictive value PPV = positive predictive value.