FIG. 1.

Analysis of genomic DNA from E. faecium HM1025 and HM1025-1, digested with SmaI (A and B, left) and from E. faecalis JH2-2 and a transconjugant digested with SfiI (A and B, right) by pulsed-field gel electrophoresis and hybridization. (A) Lanes 1, E. faecium HM1025; lanes 2, E. faecium HM1025-1. (B) Lanes 1, E. faecalis JH2-2; lanes 2, E. faecalis JH2-2/L1 (transconjugant resistant to erythromycin, lincomycin, gentamicin, streptomycin, and tetracycline). The digested fragments were transferred to a nylon sheet and hybridized to an in vitro digoxigenin-labeled linB probe. Numbers on the left of the gels are molecular sizes in kilobases.