FIG. 3.
Autoradiogram of l-[35S]methionine-labeled polypeptide specified in vitro by the amplification product of linB. (A) Protein electrophoresis in a 15% polyacrylamide gel containing sodium dodecyl sulfate. After separation, proteins were stained with Coomassie blue. (B) Visualization of labeled protein bands in the gel by autoradiography. Lanes 1, control without template; lanes 2, protein products synthesized from PCR-generated linB DNA; lanes 3, molecular mass markers (masses on the left are expressed in kilodaltons). The position of LinB (31,195 Da) is indicated.