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. 2022 Mar 15;22:119. doi: 10.1186/s12935-022-02538-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — NCAPG may activate Wnt/β-catenin signaling pathway by binding to β-catenin in CRC cell lines. A, B Western blotting assessed the expression of Wnt/β-catenin signaling pathway related proteins upon NCAPG silencing or overexpression in HCT116 and SW480 cell lines. C The expression of Wnt/β-catenin signaling pathway target factors were examined in HCT116 and SW480 cell lines by qRT-PCR. D The expression of NCAPG is correlated with CTNNB1 in CRC. E The interaction between NCAPG and β-catenin was obtained by CO-IP. F The effects of silencing or overexpression of NCAPG on the localization of β-catenin in HCT116 and SW480 cell lines. *P < 0.05, **P < 0.01 and ***P < 0.001