Fig. 2.

CYP1B1 is critical to maintaining the PCSC subpopulation in PCa. A Representative IF staining of CYP1B1 (green) and CD44 (red) in PC3 cells. Scale bar = 50 μm. B–E Colocalization of CD44 and CYP1B1 (t-test). B, D Representative IF staining of CD44 (red) and CYP1B1 (green) in LNCaP cells after the indicated treatment. Scale bar = 50 μm. D Quantification of (B). E Quantification of (C). F, G mRNA (left) and protein (right) levels of CYP1B1 and CD44 in bicalutamide- or DMSO-treated PCa cells (t-test). H Representative image and quantification of tumorsphere formation in CYP1B1 knockdown or control LNCaP-abl cells (t-test). Scale bar = 100 μm. I A representative flow cytometric analysis of the proportion of PCSCs (CD44⁺/CD24⁻) in CYP1B1 knockdown or control PCa cells. All values represent the means ± SD from three independent experiments. *p < 0.05