FIGURE 7.

Natural killer (NK)-derived interferon-γ (IFN-γ) stimulates tumour necrosis factor (TNF)-dependent macrophage cytotoxicity in the presence of benralizumab-stimulated eosinophils (Eos). a) Quantification of IFN-γ expression in NK cells upon benralizumab treatment, represented as percentage of total CD56⁺ CD94⁺ cells (n=4). b) Quantification of TNF expression in macrophages in the presence of eosinophils after stimulation with 10 ng·mL⁻¹ IFN-γ, represented as percentage of total CD163⁺ CD11b⁺ macrophages (n=4). c) Quantification of the induced expression of TNFR1 on eosinophils in the presence of macrophages after stimulation with 10 ng·mL⁻¹ IFN-γ, represented as percentage of total CD66b⁺ Siglec-8⁺ cells (n=4). Data are mean±sem. Two-way ANOVA with Tukey's multiple comparisons. ***: p<0.001, benralizumab versus parent fucosylated anti-interleukin-5 receptor α (PF anti-IL-5Rα). ⁺⁺⁺: p<0.001; ⁺⁺⁺⁺: p<0.0001, 10 ng·mL⁻¹ IFN-γ+Eos+Mac versus Eos+Mac. Statistical values are presented in supplementary table S2.