Skip to main content
. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Nat Microbiol. 2021 Dec 14;7(1):108–119. doi: 10.1038/s41564-021-01019-2

Extended Data Fig. 10. Modification enables addition of spike-ins to RNA.

Extended Data Fig. 10

a, A schematic of how to design, produce, and apply synthetic RNA spike-ins (SRSIs). b, A limited titration experiment where SRSIs of varying concentrations were added to two clinical samples with low and intermediate SARS-CoV-2 CTs. SRSIs were added to the sample at the RNA stage; the sample with a low CT (20) was then normalized to CT 25 at the cDNA stage, whereas the sample with mid CT (26) was not normalized.