Figure 6.

Biofilm and kinetics of biofilm formation of UPEC-46. (a) The biofilm formation assays were performed in different culture media (DMEM high glucose, preconditioned DMEM, and human urine) and abiotic surfaces (polystyrene and glass), with incubation for 24 h at 37°C. The dashed line represents the cutoff OD between forming and non-forming biofilm strains [49]. The cutoff was defined as three standard deviations above the mean OD of the negative control. (b) The kinetic assays were performed in DMEM high glucose or human urine culture media during different incubation periods (3, 6, 9, 12, and 24 h) on polystyrene surface at 37°C. These tests were performed in the presence or absence of 1% α-D-man. In all tests performed, EAEC 042 and E. coli DH5α were used as positive and negative controls, respectively. The assays were performed in triplicate and repeated three times. The data presented consist of the mean ± standard deviation