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Immunogold labeling of AFP and TEM analysis. (a) wild-type UPEC-46, (b) complemented mutant (UPEC-46::afpA (pPAS3)), and (c) afpA mutant (UPEC-46::afpA) were labeled with adsorbed anti-UPEC-46 serum and goat anti-rabbit IgG conjugated with 10 nm gold particles, contrasted with 2% uranyl acetate in water. (d) Wild-type strain (UPEC-46) labeled with pre-immune serum was used as negative control. Bars = 200 nm
